Hepatitis B Annual
Home

Current Issue  

Back Issues   

Instructions   

Search Login    Users online: 462 Print this page  Email this page Small font sizeDefault font sizeIncrease font size 
>>> Ahead of Print <<<


 
 Table of Contents    
REVIEW ARTICLE  
Year : 2009  |  Volume : 6  |  Issue : 1  |  Page : 110-115
Hepatitis B virus transmission and reprocessing of endoscopes


Department of Gastrointestinal Sciences, Christian Medical College and Hospital, Vellore - 632 004, India

Click here for correspondence address and email

Date of Web Publication19-Feb-2011
 

   Abstract 

Hepatitis B virus (HBV) cross-infection during endoscopy is rare. Most of the reported cases have occurred when the endoscope reprocessing was inadequate. Standard reprocessing of endoscopes and accessories is sufficient to prevent HBV transmission.

Keywords: Endoscope reprocessing, HBV transmission, standard guidelines

How to cite this article:
Dutta AK, Chacko A. Hepatitis B virus transmission and reprocessing of endoscopes. Hep B Annual 2009;6:110-5

How to cite this URL:
Dutta AK, Chacko A. Hepatitis B virus transmission and reprocessing of endoscopes. Hep B Annual [serial online] 2009 [cited 2019 Jul 18];6:110-5. Available from: http://www.hepatitisbannual.org/text.asp?2009/6/1/110/76908



   Introduction Top


Rapid technological advances have widened the array of diagnostic and therapeutic procedures performed endoscopically. As a result, the number of endoscopies performed is increasing and along with it the potential for complications. [1] Transmission of infection is an important concern as the surfaces and channels of endoscopes are exposed to body fluids and blood during endoscopy. [2] Meticulous cleaning and disinfection of endoscopes and accessories are critical in preventing cross-infection. [2] Multiple societies have laid down guidelines for disinfection of endoscopes and accessories. [3],4[] Infectious complications resulting from endoscopy are rare, with an estimated incidence of one in 1.8 million procedures, reported in the early nineties. [5] Since the introduction of appropriate disinfection guidelines, the estimated incidence has further declined to one in 10 million. [6] Evidence shows that most instances of transmission of infection, during endoscopy, results from the failure to adhere to proper disinfection guidelines. [7],[8],[9] Infectious agents reported to be transmitted during endoscopies include bacteria (Pseudomonas,  Salmonella More Details, H. pylori) and viruses (HCV, HBV). [6] This chapter focuses on the evidence of HBV transmission attributed to endoscopy and methods of prevention.


   HBV Transmission Attributed to Endoscopy Top


Transmission of HBV attributable to endoscopy is extremely rare. [5],[6] Multiple prospective studies on patients undergoing endoscopies, with instruments previously used on HBV-infected patients, have failed to show evidence of infection during the follow up-period. [10],11],[12],[13] A case control study by Villa and colleagues did not find any causal relationship between endoscopy and HBV infection. [14] In fact only five cases of HBV transmission related to endoscopy have been reported and none so far in this century. [6] Nelson et al., in their review, have suggested that in three of these five cases the reprocessing was inadequate. [6],[15],[16],[17] In the other two reports, the association with endoscopy was not properly established. [18],[19] Surveys from India and the rest of the Asia-Pacific regions have revealed that disinfection procedures for endoscopes and accessories were inadequate in many centers. [20],[21] This is a matter of serious concern as it provides the opportunity for transmission of infection.


   Endoscope Reprocessing and Prevention of HBV Transmission Top


Infection transmission during endoscopy may be exogenous (patient to patient or health care worker to patient or patient to health care worker) or endogenous (spread of infection from the mucosal surface to the blood stream and distant sites). [5] HBV transmission is exogenous. For successful transmission, the chain of infection has to be intact. This includes a viable virus present on the mucosal surface / blood / secretions in sufficient quantities, which should be carried by a suitable vehicle to a susceptible host at an appropriate portal of entry. [4] The best way to break this chain of infection is by eliminating the virus from the vehicle of transmission (endoscopes and accessories) by proper reprocessing.

There are no special reprocessing guidelines for endoscopes and accessories, to prevent HBV cross-infection. Standard reprocessing is sufficient to eliminate them. [17],[22] This is advantageous as the HBV infection status is usually unknown in many patients at the time of endoscopy. In a study from Japan, samples were obtained by flushing water in the endoscope channel after cleaning it with an enzyme / detergent and again after reprocessing with a disinfectant. [23] In the samples obtained from endoscopes used for HBV infected patients (n = 17), reverse transcriptase polymerase chain reaction (RT-PCR) for HBV DNA was performed in all, and five were positive for HBV DNA at the end of cleaning, but none after reprocessing. An animal model study on HBV transmission via invasive equipment showed that disinfection alone may not eliminate viral transmission and adequate cleaning prior to disinfection is essential. [24] The studies show that proper cleaning followed by disinfection is the key to prevent HBV transmission.

The reprocessing guidelines differ according to the device used. The classification proposed by Spaulding divides the medical devices into critical and semi-critical, based on the risk of infection with their use. [3] A device that comes into contact with the mucous membrane, but does not penetrate the sterile tissue is classified as semi-critical. The fibreoptic endoscope comes under this category. These devices have a moderate risk of infection transmission and require a high-level of disinfection (destruction of all vegetative microorganisms, mycobacteria, viruses, fungal spores, and some, but not all, bacterial spores) before reuse. [3] The steps of reprocessing include:

  1. Cleaning the endoscope and its detachable components using a detergent solution and brushes
  2. High level of disinfection using a liquid chemical germicide (e.g., 2% glutaraldehyde for ten minutes)
  3. Drying the instrument [6],[25]


A device that penetrates the skin, mucosa, body cavity or vascular space is classified as critical. This includes needles, biopsy forceps, sphincterotomes, and so on. Generally these devices are labeled as single use, but financial constraints may necessitate their reuse. A critical device has a high risk of transmitting infection and requires sterilization (destruction of all microbial life) before reuse. [3] The device should be thoroughly cleaned using enzymes, brushes, and detergent solutions. Sterilization may be done by autoclaving with steam at 134ºC for 18 minutes. Gas sterilization by using ethylene oxide or plasma is also effective. Apart from using the standard reprocessing techniques, adequate training of staff and quality control measures are also essential in preventing and monitoring infection transmission. [3]

In conclusion, HBV transmission during endoscopy is rare. Current disinfection guidelines, if followed properly, are sufficient to eliminate the risk of hepatitis B transmission.

 
   References Top

1.Jalocha L, Wojtun S, Gil J. Incidence and prevention methods of complications of gastrointestinal endoscopy procedures. Pol Merkur Lekarski 2007;22:495-8.  Back to cited text no. 1
    
2.ASGE Standards of Practice Committee, Banerjee S, Shen B, Nelson DB, Lichtenstein DR, Baron TH. Infection control during GI endoscopy. Gastrointest Endosc 2008;67:781-90.  Back to cited text no. 2
    
3.Nelson DB, Jarvis WR, Rutala WA, Foxx-Orenstein AE, Isenberg G, Dash GP, et al. Multi-society guideline for reprocessing flexible gastrointestinal endoscopes. Dis Colon Rectum 2004;47:413-20; discussion 20-1.  Back to cited text no. 3
[PUBMED]  [FULLTEXT]  
4.Beilenhoff U, Neumann CS, Rey JF, Biering H, Blum R, Cimbro M, et al. ESGE-ESGENA Guideline: Cleaning and disinfection in gastrointestinal endoscopy. Endoscopy 2008;40:939-57.  Back to cited text no. 4
[PUBMED]  [FULLTEXT]  
5.Kimmery MB, Burnett DA, Carr-Locke DL, et al. Transmission of infection by gastrointestinal endoscopy. Gastrointest Endosc 1993;39:885-8.  Back to cited text no. 5
    
6.Nelson DB, Muscarella LF. Current issues in endoscope reprocessing and infection control during gastrointestinal endoscopy. World J Gastroenterol 2006;12:3953-64.  Back to cited text no. 6
[PUBMED]  [FULLTEXT]  
7.Spach DH, Silverstein FE, Stamm WE. Transmission of infection by gastrointestinal endoscopy and bronchoscopy. Ann Intern Med 1993;118:117-28.  Back to cited text no. 7
[PUBMED]  [FULLTEXT]  
8.Ramakrishna BS. Safety of technology: Infection control standards in endoscopy. J Gastroenterol Hepatol 2002;17:361-8.  Back to cited text no. 8
[PUBMED]  [FULLTEXT]  
9.Morris J, Duckworth GJ, Ridgway GL. Gastrointestinal endoscopy decontamination failure and the risk of transmission of blood-borne viruses: A review. J Hosp Infect 2006;63:1-13.  Back to cited text no. 9
[PUBMED]  [FULLTEXT]  
10.McClelland DB, Burrell CJ, Tonkin RW, Heading RC. Hepatitis B: Absence of transmission by gastrointestinal endoscopy. Br Med J 1978;1:23-4.  Back to cited text no. 10
[PUBMED]  [FULLTEXT]  
11.Moncada RE, Denes AE, Berquist KR, Fields HA, Murphy BL, Maynard JE. Inadvertent exposure of endoscopy patients to viral hepatitis B. Gastrointest Endosc 1978;24:231-2.  Back to cited text no. 11
[PUBMED]    
12.Chiaramonte M, Farini R, Truscia D, Zampieri L, Di Mario F, Pornaro E, et al. Risk of hepatitis B virus infection following upper gastrointestinal endoscopy: A prospective study in an endemic area. Hepatogastroenterology 1983;30:189-91.  Back to cited text no. 12
[PUBMED]    
13.Koretz RL, Camacho R. Failure of endoscopic transmission of hepatitis B. Dig Dis Sci 1979;24:21-4.  Back to cited text no. 13
[PUBMED]    
14.Villa E, Pasquinelli C, Rigo G, Ferrari A, Perini M, Ferretti I, et al.. Gastrointestinal endoscopy and HBV infection: No evidence for a causal relationship: A prospective controlled study. Gastrointest Endosc 1984;30:15-7.  Back to cited text no. 14
[PUBMED]    
15.Birnie GG, Quigley EM, Clements GB, Follet EA, Watkinson G. Endoscopic transmission of hepatitis B virus. Gut 1983;24:171-4.  Back to cited text no. 15
[PUBMED]  [FULLTEXT]  
16.Morris IM, Cattle DS, Smits BJ. Endoscopy and transmission of hepatitis B. Lancet 1975;2:1152.  Back to cited text no. 16
    
17.Seefeld U, Bansky G, Jaeger M, Schmid M. Prevention of hepatitis B virus transmission by the gastrointestinal fibrescope: Successful disinfection with an aldehyde liquid. Endoscopy 1981;13:238-9.  Back to cited text no. 17
[PUBMED]  [FULLTEXT]  
18.Davis AR, Pink JM, Kowalik AM, Wylie BR, McCaughan GW. Multiple endoscopies in a Sydney blood donor found positive for hepatitis B and C antibodies. Med J Aust 1996;164:571.  Back to cited text no. 18
    
19.Federman DG, Kirsner RS. Leukocytoclastic vasculitis, hepatitis B, and the risk of endoscopy. Cutis 1999;63:86-7.  Back to cited text no. 19
[PUBMED]    
20.Arora A, Seth S, Tandon RK. Gastrointestinal endoscope disinfection practices in India: Results of a national survey. Indian J Gastroenterol 1992;11:62-4.  Back to cited text no. 20
[PUBMED]    
21.Ahuja V, Tandon RK. Survey of gastrointestinal endoscope disinfection and accessory reprocessing practices in the Asia-Pacific region. J Gastroenterol Hepatol 2000;15:G78-81.  Back to cited text no. 21
[PUBMED]  [FULLTEXT]  
22.Bisset L, Cossart YE, Selby W, West R, Catterson D, O'hara K, et al. A prospective study of the efficacy of routine decontamination for gastrointestinal endoscopes and the risk factors for failure. Am J Infect Control 2006;34:274-80.  Back to cited text no. 22
[PUBMED]  [FULLTEXT]  
23.Ishino Y, Ido K, Sugano K. Contamination with hepatitis B virus DNA in gastrointestinal endoscope channels: Risk of infection on reuse after on-site cleaning. Endoscopy 2005;37:548-51.  Back to cited text no. 23
[PUBMED]  [FULLTEXT]  
24.Chaufour X, Deva AK, Vickery K, Zou J, Kumaradeva P, White GH, et al. Evaluation of disinfection and sterilization of reusable angioscopes with the duck hepatitis B model. J Vasc Surg 1999;30:277-82.  Back to cited text no. 24
[PUBMED]  [FULLTEXT]  
25.Tandon RK. Disinfection of gastrointestinal endoscopes and accessories. J Gastroenterol Hepatol 2000;15:G69-72.  Back to cited text no. 25
[PUBMED]  [FULLTEXT]  

Top
Correspondence Address:
Ashok Chacko
Department of Gastrointestinal Sciences, Christian Medical College and Hospital, Vellore
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0972-9747.76908

Rights and Permissions




 

Top
 
  Search
 
    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
    Email Alert *
    Add to My List *
* Registration required (free)  


    Abstract
    Introduction
    HBV Transmission...
    Endoscope Reproc...
    References

 Article Access Statistics
    Viewed5618    
    Printed353    
    Emailed1    
    PDF Downloaded258    
    Comments [Add]    

Recommend this journal